3rd UF Water Institute Symposium Abstract

Submitter's Name Reyna Colli-Dula
Session Name Posters - Nutrient dynamics and enrichment impacts in aquatic ecosystems 2
Category Nutrient dynamics and enrichment impacts in aquatic ecosystems
Poster Number 45
Author(s) Reyna Cristina Colli-Dula,  University of Florida (Presenting Author)
  Alvine Mehinto,  University of Florida
  Kevin J. Kroll, University of Floria
  David  Barber, University of Florida
  Nancy  Denslow, University of Florida
  Understanding of Benzene and Trichloroethylene Effects in Largemouth Bass Using Microarrays
  Benzene and trichloroethylene (TCE), known environmental pollutants, have been detected in aquatic systems causing adverse effects in fish. This is due to high usage and release of both industrial discharges and releases during oil extraction and transport as well as to high mobility of these compounds in the atmosphere. One way to explore mechanisms of toxicity of these compounds is through quantification of gene expression in fish. We used microarrays to investigate changes in gene expression caused by exposure to benzene and TCE in largemouth bass. Twelve fish were injected in each group for vehicle control as well as for each treatment (using a single dose of benzene or TCE 10 mg/kg b/w). After injection, the fish were sacrificed at 48h. Livers and gonads were obtained and analyzed for gene expression changes using microarrays. The hypothesis was that concentrations used for benzene and TCE would produce changes in sensitive genes. Resulting ANOVA analysis in the liver revealed 1272 and 1116 genes were significantly affected (p